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INFLUENCE OF FISH COLLAGEN ON VISCOELASTIC PROPERTIES AND SOL-GEL PHASE TRANSITION OF CHITOSAN SOLUTIONS

Date added: 2018-04-03
Type: Article

Author / authors

Piotr Owczarz

Piotr Owczarz

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Anna Rył

Anna Rył

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Abstract

The thermosensitive hydrogels are widely used in tissue engineering due to their non-invasive application.
Special interest of researchers, due to the specific characteristics of both materials, is aimed at composites
of natural origin obtained from chitosan hydrogels combined with collagen. The mechanical properties of the
thermosensitive chitosan-fish collagen hydrogels and the sol-gel phase transition parameters were determined
by the rotational rheometry measurement techniques. Based on comparison of the obtained storage modulus
G' curves, it was found that the addition of collagen negatively affects the mechanical properties of composite
scaffolds. The addition of this protein substance decreases their elasticity. Only the smallest concentration
(0.25g collagen/1 g chitosan) of collagen improves the mechanical properties of composite hydrogels, from 56
kPa to 61 kPa. Conducted non-isothermal studies allowed to conclude that the addition of collagen causes an
increasing temperature of sol-gel phase transition. However, the observed changes are not a monotone
function of the biopolymer concentration.

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